Ag4CL3 Related to Lignin Synthesis in Apium graveolens L.

4-Coumarate: coenzyme A ligase (4CL; EC 6.2.1.12) is an important enzyme in the phenylpropanoid metabolic pathway that controls biosynthesis of lignin and flavonoids. In this study, to identify function Ag4CL3 gene celery, was cloned from celery cv. “Nanxuan Liuhe Ziqin”. Sequence analysis results showed contained open reading frame (ORF) with a length 1688 bp, 555 amino acids were encoded. The protein highly conserved among different plant species. Phylogenetic demonstrated 4CL proteins carrot belonged same clade. mainly composed 31.89% α-helixes, 18.02% extended strands, 6.67% β-turns, 43.42% random coils, signal peptide unfound. total 62 phosphorylation sites class-I superfamily adenylate-forming domains found. As growth time increased, height stem thickness also petiole content increased became lignified gradually. relative expression levels Ziqin” petioles higher than those other tissues, highest level occurring 70 d after sowing. contents transgenic Arabidopsis thaliana lines hosting WT. overexpression led significant upregulation A. plants, except for AtPAL, AtCCR, AtLAC. This study speculates genes are related synthesis graveolens.